RNA

Part:BBa_K2361003:Design

Designed by: Thijs Ettema   Group: iGEM17_Groningen   (2017-10-10)


pUSP45 tracrRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is essential for a functional CRISPR system, as the crRNA:tracrRNA;dCas9 complex is needed for signalling.


Source

The promoter sequence, together with the tracr RNA was obtained by synthesis based on designs from the team.

References